GnRH类似物诱发家兔排卵效果的研究

The Effect of GnRH Analogue Inducing Ovalution in Rabbits

利用国产GnRH类似物(LRH-A_2),对4个不同品种的家兔:新西兰兔、加利福尼亚兔、西德大白兔、塞北兔进行诱发排卵处理,然后人工授精,以12天时的受胎率作为比较各处理效果的标准。结果表明:静脉注射5μg、10μg、15μg、20μgLRH-A_2后输精的受胎平分别为42.2％、46.0％、33.6％、30.0％,前二者与后二者存在显著差异(P>0.01),但前二者之间、后二者之间差异不显著(P>0.05);在输精前的0、3、6小时注射LRH-A_2,相应的受胎率分别为46.0％、40.0％、42.2％,差异不显著(P>0.05);不同注射方式的受胎率有显著差异(P<0.01),肌肉注射的受胎率高于静脉注射的受胎率(57.8％vs.46.0％,P<0.01),但与皮下注射的受胎率差异不显著(P>0.05),而皮下注射的受胎率与静脉注射的受胎率差异也不显著(41.9％vs.46.0％,P>0.05)。根据试验结果可以得出结论:GnRH类似物(LRH-A_2)诱发家兔排卵的效果与其处理剂量和方式有密切关系,是一种比较理想的家兔诱发排卵药物。

GnRH analogue ( LRH- A2) was used for inducing ovalution in four breeds of rabbits: New Zealand, California, SAB and Saibei, and the efiect of various treatments was evaluated by the conception rate (CR) of rabbits on 12th day after insemination. In experiment 1, the rabbits were treated with 5, 10, 15, 20μg LRH-A2 at the time of artificial insemination ( AI). The conception rate was higher for treated rabbits with 5, 10μg, than that with 15, 20μg (42.2%, 46.0%vs.33.6%, 30.0%, P<0.01) . In experiment 2, injecting LRH-A2 at 0, 3, 6h before AI, the CR of rabbits with different injecting was similar (P > 0.05) . In experiment 3, animals were injected in three methods, the intramuscular injection group had a greater CR than the intravenous injection group (57.8%vs.46.0%, P<0.01), but the CR of subcutaneous injection group was not significantly different from either of the two groups (41.9%vs.57.8%, 41.9%vs.46.0%, P>0.05) . These results showed that the effect of GnRH analogue (LRH-A2) inducing ovalution is related closely with the dose and method of treatments, and LRH-A2 is an ideal stimulating agent for rabbits.