体内诱导艾氏腹水瘤细胞的耐药性研究

A Research of Inducing Multidrug Resistance of EHR Cells in Vivo

肿瘤细胞耐药性的存在是临床化疗失败的主要原因之一。本实验在小鼠体内用阿霉素（ADR）诱导艾氏腹水瘤细胞（EHR）的耐药性，探讨细胞产生耐药性的机理。HPLC法测定细胞内药物浓度．结果表明耐药细胞─—EHR/ADR细胞内ADR积聚低于EHR细胞，而对ADR外排快于EHR细胞；异博定（VER）增加EHR／ADR细胞对ADR的摄取并阻滞其外排．而对EHR影响不大，揭示EHR／ADR细胞具有MDR特性。

One of the major causes for failure of chemotherapy is the development of multidrug resistance (MDR ). In order to study the mechanism of MDR, we established MDR cells induced by ADR in vivo. Intracellular Contant of ADR was determined by HPLC.The results showed that ADR accumulated in EHR /ADR cells was less than that in EHR cells.And extrude more ADR in EHR/ADR cells than in EHR cells VER increased ARD intake and blocked its extrusion in ENR /ADR cells .while there was little effect of VER on drug intaking and extrusion in EHR cells. It showed that EHR /ADR cells were characteristic of MDR.