激素对枸杞体细胞胚发生及可溶性蛋白质含量和组分的影响

EFFECT OF PLANT HORMONEAND THE CHANGES OF SOLUBLE PROTEINS ON SOMATIC EMBRYOGENESIS FROM LYCIUM BARBARUM

以宁夏枸杞无菌苗叶片为材料，对体细胞胚发生过程中激素作用、可溶性蛋白质变化及体细胞胚发生频率进行了研究。发现不同激素处理、可溶性蛋白质含量、组分和体细胞胚发生频率均有一定的差异，三者之间存在着相关性。结果如下：（１）ＭＳ＿１、ＭＳ＿２和ＭＳ＿３三种培养基上继代的愈伤组织难以诱导形成体细胞胚，为非胚性愈伤组织；而从ＭＳ＿１、ＭＳ＿２和ＭＳ＿３分别转到ＭＳ＿０和ＭＳ＿４培养基上的愈伤组织体细胞胚发生频率高，属于胚性愈伤组织。其中以ＭＳ＿３转至ＭＳ＿０后的体细胞胚发生频率最高。（２）可溶性蛋白质ＳＤＳ－ＰＡＧＥ分析表明：非胚性愈伤组织有特异性蛋白质６７ｋＤ，胚性愈伤组织有特异性蛋白质３５ｋＤ和４４ｋＤ；蛋白质３３ｋＤ和６７ｋＤ受２，４－Ｄ调控，蛋白质４９ｋＤ、５７ｋＤ受６ＢＡ调控，而蛋白质３７ｋＤ、５１ｋＤ受２，４－Ｄ和６ＢＡ协同调控。

Bystudy on the effect of plant hormone,the changes of soluble proteins and the frequencyof somatic embryogenesis from Lycium barbarum,it was found that there may be certain relation-ship between them,The result showed that the frequency of somatic embryogenesis was the high-est when the callus was cultured with MS_0 medium after cultured with MS_3 medium. The SDS-PAGE patterns showed that non-embryonic callus predticed specific protein 67 kD,and embry-onic calltis produced specific proteins 35 kD and 44 kD.Inaddition,2,4-D regulated the genera-tion of proteins 33 kD and 67 kD,6 BA regulated the generation of proteins 49kD and 57kD,and proteins 37kD and 51kD were coordinately regulated by 2,4-Dand 6BA.