摘要
应用免疫荧光抗体技术检测了整合有猪瘟病毒反义基因的PK-15细胞克隆对猪瘟病毒的抑制效应。结果表明,五个不同的反义基因片段对猪瘟病毒的抑制效率存在很大差异,其中A片段的抑制效率最高(94%~98%),B片段次之(58%~76%),C片段再次(~64%),D片段和E片段未见明显的抑制效应。抑制效率的差异可能与反义基因片段的位置、长短以及反义RNA表达质粒载体的差异有关。
The specific inhibition of hog chloera virus (HCV)replication by antisense RNA expressed in cells transfected vrith HCV antisense gene fragments was demonstrated by antibody(IFA)at the presence of Zn2+ (50 or 65 fig/Das inducer of MT promoter. The inhibition rate in mMT/HCA,mMT/HCB and mMT/HCC transfected cell clones were 94%-98%-58%-76%, and^64% respectively. No apparent inhibition was observed in pMT/HCD and pMT/HCE transfected clones. The reasons for the differences in inhibition effects may be resulted from the sizes and positions of the HCV antisense gene fragments or the asRNA expressing vectors.
出处
《生物技术通讯》
CAS
1995年第2期62-65,共4页
Letters in Biotechnology
基金
国家863计划
国家自然科学基金
关键词
猪瘟病毒
反义基因
病毒抑制
Hog cholera virus, Antigene, Virus inhibition