猪瘟病毒反义基因在培养细胞上的效应研究——反义基因对猪瘟病毒的抑制作用

Study on the Effects of Hog Cholera Virus Antisense Gene Fragments In Vitro ——The specific Inhibition of hog Cholera Virus by Its Antigene Fragments

应用免疫荧光抗体技术检测了整合有猪瘟病毒反义基因的PK-15细胞克隆对猪瘟病毒的抑制效应。结果表明,五个不同的反义基因片段对猪瘟病毒的抑制效率存在很大差异,其中A片段的抑制效率最高(94％～98％),B片段次之(58％～76％),C片段再次(～64％),D片段和E片段未见明显的抑制效应。抑制效率的差异可能与反义基因片段的位置、长短以及反义RNA表达质粒载体的差异有关。

The specific inhibition of hog chloera virus (HCV)replication by antisense RNA expressed in cells transfected vrith HCV antisense gene fragments was demonstrated by antibody(IFA)at the presence of Zn2+ (50 or 65 fig/Das inducer of MT promoter. The inhibition rate in mMT/HCA,mMT/HCB and mMT/HCC transfected cell clones were 94%-98%-58%-76%, and^64% respectively. No apparent inhibition was observed in pMT/HCD and pMT/HCE transfected clones. The reasons for the differences in inhibition effects may be resulted from the sizes and positions of the HCV antisense gene fragments or the asRNA expressing vectors.