单胺类神经递质在大鼠急性肝性脑病发生中的作用

The Role of monoamines in the pathogenesis of acute hepatic encephalopathy in rats

目的:阐明单胺类神经递质在大鼠急性肝性脑病发生中的作用。方法:采用硫代乙酰胺所致大鼠(n=10)急性肝功能衰竭模型,测定血浆及脑组织中单胺类神经递质与血浆内毒素含量,并与正常组(n=5)进行比较。结果:在肝性脑病进入Ⅲ-Ⅳ期时,脑组织中仅5-羟色胺含量明显升高(5 139±48.0比394.7±43.8ng/g),血浆5-羟色胺升高幅度是脑组织中的两倍并伴严重内毒素血症。结论:5-羟色胺可能是肝性脑病时昏迷的因素,脑组织中升高的5-羟色胺部分源于血浆。

AIM To investigate the role of monoamines in the pathogenisis of acute hepatic encephalopathy.METHODS Monoamines were measured both in brain fissure and plasma on thioacetamide-induced acute hepaticencephalopathy,and plasma endotoxin was measured as ewll in rats.RESULTS In brain tissure only serotonin was significently increased in stage Ⅲ-Ⅳ hepatic encephalopahy compared withstage Ⅰ-Ⅱ)513.9±48.0ng/g vs 423.5±23.4 ng/g.x±s).Plasma serotion was increased more than that in brain andaccompanied by endotoxinmia.CONCLUSION Increased serotonin in brain tissure,probaly partly from plasma,is the main factor in the development of hepatic encephalopathy from slight neuropsychic symptom to coma.