表皮生长因子及谷氨酰胺对大鼠乙酸性结肠炎保护作用的病理学研究

Cytoprotection of epidermal growth factor and glutamine on acetic acid - induced colitis in rats

目的:本文肌注表皮生长因子EGF和(或)饲喂谷氨酰胺GLN防治大鼠乙酸性结肠炎。方法:Sprague-Dawley系大鼠体重200—220g,40只分为乙酸组、EGF组、GLN组、EGF+GLN组各10只,从大体.光镜、电镜(透射、扫描)观察结肠粘膜的损伤情况。结果:上述4组结肠粘膜损害的指数分别为3.11±0.93,1.78±0.97,1.30±0.48及1.10±0.32(P<0.01)。镜下观察也有明显疗效,以EGF+GLN组为最佳。结论:EGF及GLN对结肠粘膜细胞形态结构具有保护作用,且优于两者单独作用,这为表皮生长因子、谷氨酰胺用于防治人类溃疡性结肠炎提供了理论依据。

AIM To study on the mechanism of epidermal growth factor)EGF)im and/or glutamine)GLN)to protect acetic acid-induced colitis in rats.METHODS Sprague-Dawley rats,(n=40,body weight,200-220g)were randomly divided into four groups,i.e.acetic acidgroup,EGF group,glutamine group and combined EGF and GLN group.The colonic mucosal damage was examined macroscopically(0-4 degrees),microscopically and election microscopically.RESULTS The damaged indices in colonic mucosal pathology were 3.11±0.93)acetic acid group),1.78±0.97(P<0.01,EGF group),1.30±0.48(P<0.01,GLN group)and 1.10 + 0.32)P<0.01,EGF + GLN group)respectively.Whencombining EGF and GLN was used,the mucosal structure tended to normal.CONCLUSION The results suggest that combining EGF and GLN has remarkly cytoprotective effect on colonic mucosa,which provides theoretic basis to prevent and treat human ulcerative colitis.