LAK细胞治疗对肝癌患者免疫功能影响的研究

Effects of LAK cell treatment on immunologic function in oatients with liver cancer

目的:研究LAK细胞治疗前后免疫功能的改变。方法:应用人胚胎脾脏及胸腺制备同种异体LAK细胞治疗晚期肝癌30例。在治疗前及治疗10d后检测末梢T细胞亚群、mIL—2R表达(间接免疫荧光法).NK细胞活性(^(125)I—UdR释放法)及血清sIL-2R水平(ELISA法)。结果:治疗后患者末梢血CD_4/CD_8比值增加(1.11± 0.24→1.31±0.25,P<0.01)NK细胞活性提高(36.4％→24.3％→47.6％±25.1％,P<0.01)、mIL-2R表达阳性率增高(33.3％±7.2％→±39.5％±8.4％,P<0.05)、而血清中sIL-2R含量降低[280.8±96.5→248.4±89.4(×10~3U/L)P<0.05]。结论:LAK细胞治疗可增强肝癌患者的免疫功能。

AIM The changes of immunologic function were studied by using LAK cells.METHODS The LAK cells were prepared from the spleen and thymus gland of the fetus.Before and after 10 days oftreatment,the T cell subsets,NK cell activity,the expression of mIL-2R in peripheral blood and the level of serum sIL-2R in 30 patients with advanced liver cancer were detected.The T cell subbsets and the expression of mIL-2R weredetected by indirect immunofluorescence technique.The NK cell activity was determined by 1251-UdR release assay andthe serum level of sIL-2R was studied by EL1SA.RESULTS After the treatment of LAK cells,the CD4/CD8 ratio was increased)1.1±0.24→1.31±0.25,P<0.01),theNK cell activity was increased)36.4%±24.3%→47.6%±25.1%,P<0.01),the expression of the mIL-2R was increased(33.3%±7.2%→39.5%±8.4%,P<0.05)and the level of serum sIL-2R was decreased)280.8 ±96.5→248.4±89.4)×103 U/L),P<0.05)in liver cancer patients.CONCLUSION The treatment with LAK cells can improve the immunologic function of the patients with liver cancer.