肝细胞再生刺激物质长期使用对慢性肝损害肝细胞倍体影响

Effect of hepatic stimulator substance on DNA - ploydies in chronic CC14 - induced liver damage

目的:探讨肝细胞再生刺激物质(HSS)长期使用,对正常机体及病变组织细胞有无致畸.致癌作用。方法:采用从新生小牛肝中研制的HSS,对慢性肝损历动物模型进行长期治疗,利用同位素~3H-TdR掺入和流式细胞换术测定肝细胞增殖周期和倍体改变。结果:HSS①体内、外均能刺激肝细胞DNA合成(P【0.01);②增生的肝细胞增殖周期以GO/1期为主,但S期明显活跃(P】0 05);③增生肝细胞DNA倍体以4、8倍体等整倍体细胞为主,未见异常非整倍体细胞。结论:HSS长期使用无明显致畸、致癌作用。

AIM Our aim is to study whether the long term use of hepatic stimulator substance(HSS)leads to liver cell deformity orcarcinogensis.METHODS HSS was prepared from liver of calf and was used to treat CC14-induced chronic liver damage.The DNA incorporationand ploydies were measured by 3H-thymidine and flow cytometry respectively.RESULTS(1)The DNA incorporation with 3H-TdR of liver parenchymal cell in HSS-treated group increased in vivo and in vitrocompared with the control group(P<0.01).(2)The periods of proliferation cell of HSS-treated group were mainly Go/1;(3)Theploydies of HSS-treated group were mainly consisted of four,eight ploydies,and abnormal ploydies weren't found.CONCLUSIONS The long term use of HSS doesn't lead to liver cell deformity or carcinogensis.