高温对人宫颈癌细胞系（Hela）生物学和生物化学作用的实验研究

Experimental studies on effect of hyperthermia and/or irradiation on human cervical carcinoma cell line(Hela-S3)

探讨了高温或（及）辐射对Ｈｅｌａ细胞的作用，并测定其在不同实验条件下的生物学指标（生长曲线、集落形成率、分裂指数）和生化指标（核ＤＮＡ和培养液中Ｋ＋浓度）的动态变化。结果显示，高温组中４３．５℃和４５．５℃对Ｈｅｌａ细胞有明显的杀伤效应，呈对数杀伤效应；４２．５℃与４３．５℃分别于９０ｍｉｎ和１８０ｍｉｎ出现了热耐受，而４５．５℃未出现；其分裂指数呈抑制－回升－再抑制－再回升的曲线。推测是细胞同步化或再分布的结果。高温抑制细胞的有丝分裂比辐射强，而且恢复得慢，但相应的杀伤能力却低于辐射。对核ＤＮＡ定量测定结果显示，高温抑制了核ＤＮＡ的合成，且与温度呈正相关；辐射亦抑制ＤＮＡ的合成，二者联用具有协同作用，这种作用是可逆的，二者联用有降低其可逆性的作用。各实验处理组培养液中Ｋ＋浓度均有不同程度增高，以二者联用组最高。

Our studies aimed at investigating the effect of hyperthermia and/or irradiation on the Helacell both biologically and biochemically. We found that hyperthermia inhibited cell proliferation better than irradiation, while irradiation,on the other hand, was more lethal than hyperthermia. The combination of the two methods resulting in a greater cytotoxicity than could be accounted for by the addition of the cytotoxic effects of either agent employed alone, as there were synergistic interaction between them.Cytophotometry showed that the synthesis of DNA was also inhibited by hyperthermia and/or irradiation, the mean Fueigen density with or without irradiation was statistically significant ( P<0.001 ) . There may be the hyperthermia inhibited the division of the cells and then foriried heteropolyploid cells. From the DNA distribution histogram, we concluded that the decrease of DNA value in the hyperthermia combined irradiation group was primarily resulted from the decrease of the number cells which contained heteropolyploid DNA amount . K+concentration of the medium rose significantly after heating or irradiation, especially in case of combination of the two methods,as dramatic synergism. There were statistically significance each other (P<0.001 ) . This showed hyperthermia and irradiation were synergic in inhibiting the Na+ pump function in the Hela cells. From these, we consider that hyperthermia primarily disrupts tho membrane's structure and function.