脐血混合移植的基础和临床研究

Basic and clinical research on multicord blood transplantation

用单层琼脂法进行单一和混合脐血粒－单造血祖细胞（ＣＦＵ－ＧＭ）培养，结果发现不同脐血单个核细胞（ＭＮＣ）间可能互为造血细胞集落刺激活性，混合培养ＣＦＵ一ＧＭ产率明显高于单独培养之和的均值（Ｐ＜０．０５）。长期深低温保存可累积脐血造血祖细胞数量，且可降低Ｔ４淋巴细胞的抗原表达，有利减轻移植排斥。在实验基础上，对２例晚期盆腔肿瘤患儿进行了强力化疗和混合脐血移植术，病情均于一个月内完全缓解，骨髓造血重建，为造血组织移植开拓了新的供源，可能使更多的不治之症患者获得治疗的机会。

The purpose of this study was to evaluate the possibility of multicord blood transplantation. Our in vitro study showed that CFU-GM colonies in mixculture were higher than the average value of that in single cultures implying without rejection between different cord blood MNCs. On the other hand,our results revealed that long term cryopreservation could reduce the antigen markers of OKT4 cells which was generally considered to be related to GVHD. It therefore seeilis reasonable to assume that multi-cord blood transfusion would not present serious problems. Thus two patients with advanced pelvic carcinoma were treated by intensive chemotherapy with HLA mismatched multi-cord blood transplantation. The hemopoiesis of bone marrow was reconstituted and the patients were roached in complete remission within 1 month following treatment. Our preliminary results were encouraging.