宫颈癌病变组织人乳头瘤病毒感染率的免疫细胞化学与DNA杂交的比较观察

COMPARISON OF FREQUENCY OF HPV INFECTION DETECTED BY PAP METHOD WITH THAT FOUND BY DNA HYBRIDIZATION TECHNIQUE

近年来人乳头瘤病毒（ＨＰＶ）被认为与宫颈癌的发生有密切关系。该实验报道了用分子杂交技术（在严格条件下）检测了宫颈活检组织：慢性宫颈炎、宫颈上皮内瘤（ＣＩＮ）、宫颈癌和宫颈正常组织中１６型乳头瘤病毒（ＨＰＶ－１６）ＤＮＡ的阳性率，并将其与用免疫细胞化学方法（ＰＡＰ）所得结果进行了比较。结果显示１３％（２／１５）的慢性宫颈炎、１７％（２／１２）的ＣＩＮ、５６％（５１／９１）的宫颈癌含有ＨＰＶ－１６ＤＮＡ，而正常组织为阴性；ＨＰＶ属抗原仅见于３５％（６／１７）的ＣＩＮ，其他受检组织均为阴性。结果提示用ＰＡＰ法检查ＨＰＶ－１６的抗原，所得阳性率不及ＤＮＡ杂交所得阳性率高；但它作为一种简便、快速的方法用于福尔马林固定、石腊切片材料，显示ＨＰＶ属抗原，借以筛选多种不同型的ＨＰＶ对ＣＩＮ或其他组织中ＨＰＶ的增殖性感染有一定的价值。

Recently, it has been widely recognized that human papilloma virus (HPV) infection plays an important role in the etiology of cervical cancer. The frequency of HPV--16 DVA detected in cervical biopsies with chronic cervjcitis, cervical intraepithelial neoplasia (CIN) and cervical cancer was investigated with a dot blot DNA hybridization under stringent conditions and compared with that of the detection of HPV genus--specific structural antigens by an immunocytochemical method PAP on formalin--fixed, paraffin--embeded tissues. The results showed that 2 (13%) out of 15 chronic cervicitis, 2 (17 % ) out of 12 CIN, 51 (56%) out of 91 cervical cancer were postive for HPV--16 DNA and the normal samples were negative. The antigens of HPV were only found in 6 (35%) out of 17CIN, no case of chronic cervicitis or cervical cancer revealed postive antigen staining. The recults suggested that the immunocytochemical method PAP can be used as a simple and rapid screening technique for routinely processed material to detect HPVs infection which is suspected in CIN and some other tissues.