大蒜油抗肿瘤作用的流式细胞光度术分析

ANALYSIS OF GARLIC OIL ANTITUMOR EFFECT WITH FLOW CYTOMETRY

用流式细胞光度术（ｆｌｏｗｅｙｔｏｍｅｔｒｙ，ＦＣＭ）+Ｓ０．２ｍｌ／日。吐温对照组ｌＰ吐温８００．２ｍｌ／日，以排除大蒜油制剂中溶剂（吐温８０）的影响。实验组ｌＰＧＯ１００ｍｇ／ｋｇ／．日。于连续４次注射后的６ｈ，２、３、５、１０天及１０次注射ＧＯ后７天取材，行ＦＣＭ样品制备。三、ＦＣＭ样品制备与测定中国中医研究院基础所腹水癌细胞用ＰＢＳ洗涤，７０％酒精固定，ＲＮａｓｅ消化细胞中的ＲＮＡ，ＰＩ染色。用腹腔内淋巴细胞作标准二倍体细胞。用４２０型荧光激活细胞分选仪（美国Ｂｅｃｔｏ－Ｄｉｃｋｓｏｎ公司产品）测单个肿瘤细胞的ＤＮＡ含量，用组方图显示肿瘤细胞ＤＮＡ倍体性质。图中第一个是ＤＮＡ二倍体（ＺＣ）峰，处于该峰的细胞为Ｇ；／Ｇ。期细胞，第二个峰为４ＣＤＮＡ峰，该处细胞为Ｇ。－Ｉ－－Ｍ期细胞，从２。到４。之间的细胞为Ｓ期细胞。大于４。ＤＮＡ峰细胞为高倍体细胞‘’：。根据不同峰值大小判定用药后肿瘤细胞倍体性质的变化。结果ＮＳ与吐温对照组肿瘤细胞都为非整倍体性，且呈多倍体性（图１，２）。连续４次给药后６ｈ，绝大部分多倍体肿瘤细胞被杀伤，残存肿瘤细胞以二倍体为主，多倍体肿瘤细胞峰值显著下降。

By using flow cytometry (FCM) it was demonstrated that S180 ascites tumor cells implanted in the abdomen of normal mice were aneuploid, especially multiploid. After the mice were treated by inttaperitoneal injection with garlic oil, the amount of the multiploid tumor cells was reduced significantly. The histogram showed a shife to diploid, but the survival tumor cells started to divide and multiploid tumor cells increased gradually after withdrawal The regeneration of tumor cells was inhibited by injection intraperitoneally for10 day.