α—淀粉酶的反相高效液相色谱测定

Analysis of α-Amylase by RP-HPLC

本文介绍了α—淀粉酶的反相高效液相色谱的测定方法、填料和流动相的选择以及柱温的影响。采用Ｃ８、３００Ａ的填料和水～乙腈～三氟乙酸作流动相、２１０ｎｍ检测。在测定范围内浓度与响应值之间关系为：Ｃ＝０．１６４２＋１．６×１０－７Ｒ．

The reversed-phase high performance liquid chromatography (RP-HPLC) was characterized for assaing α-amylase in this peper. The Nucleosil C8 300A packing was accepted to be a stationaryphase and the water-acetonitrile-trifluoroacetic acid were used as a mobile phase. The α-amylase was sensitively detected by means of RP-HPLC, and then a liner fuction is presented as follow: C=0. 1642+1. 6*10-7R.