异质性荧光染色的巨噬细胞功能研究I．异质性荧光染色的巨噬细胞吞噬活性

INVESTIGATION ON THE FUNCTIONS OF MACROPHAGES WITH HETEROGENEOUS FLUORESCENT STAINING I. PHAGOCYTIC ACTIVITY OF THE MACROPHAGES WITH HETEROGENEOUS FLUORESCENT STAINING

利用淀粉多糖和免疫促进剂（白喉类毒素和卡介苗）诱导和活化小鼠腹腔巨噬细胞，观察了四种异质性荧光染色的巨噬细胞非特异性和特异性吞噬活性。实验证明，深蓝色和淡蓝色荧光的巨噬细胞是分化程度低的幼稚细胞，非特异性吞噬功能较弱，但在特异性吞噬过程中呈现了活跃的吞噬活性，特别是在免疫促进剂的活化下，它们的特异性吞噬功能显著增强、淡蓝绿色荧光的巨噬细胞是分化程度较高、非特异性和特异性吞噬功能最旺盛的巨噬细胞，而黄色荧光的巨噬细胞是分化程度最高、特异性吞噬功能较减退的巨噬细胞。

Nonspecific and specific phagocytic activities of macrophages with heterogeneous fluorescent staining were observed using starch polysaccharide and immune activators (diphtheria toxoid and BCG) to induce and activate mouse peritoneal macrophages. The experiments showed that dark blue and bluish fluorescent macrophages are young cells with low degree of differentiation, nonspecific phagocytic activity is weak, though they show active phagocytic activity in the process of specific phagocytosis, and their specific phagocytic function increases conspicuously especially under the actions of induction and activation of immune activators bluish-green fluorescent macrophages are higher in the degree of differentiation, being macrophages of strongest nonspecific and specific phagocytic functions. Yellow fluorescent macrophages are highest in the degree of differentiation with more weakenedfunction of specific phagocytosis.