颗粒子宫腺细胞的分离、纯化与细胞化学研究

ISOLATION,PURIFICATION AND CYTOCHEMISTRY OF THE GRANULATED METRIAL GLAND CELLS

胰蛋白酶消化小鼠子宫腺细胞区，制成单细胞悬液，滴片后进行细胞学及细胞化学研究。结果：有多种类型细胞存在于悬液中，其中中等大小细胞（直径２０μｍ左右）大部分为颗粒子宫腺细胞（ＧｒａｎｕｌａｔｅｄＭｅｔｒｉａｌＧｌａｎｄＣｅｌｌｓ，ＧＭＧｃｅｌｌｓ）。ＧＭＧ细胞约占所有细胞的５０％。利用Ｐｅｒｃｏｌｌ非连续性密度梯度离心可以提高ＧＭＧ细胞的纯度，使之达８０％以上。细胞化学研究显示ＧＭＧ细胞含ＡＣＰ，ＮＳＥ，ＬＮＡｓｅ及糖蛋白成份。

Cells of the metrial glands of mice were isolated by trypsin digestion. Cytochemistry study showed that the separated cells suspension contained 50% of granulated metrial gland (GMG) cells with diameter of 20 um, which were positive of glycogen, glycoprotein, acidphosphatase (ACP), non-specific esterase (NSE) and leucine aminopetidase (LNAse). A subsequent percoll step gradient centrifugation could improve the pulity of isolated GMG cells up to more than 80%.