大鼠内脏大神经电刺激后C－Fos在中枢神经系统中的表达

EXPRESSION OF FOS IN RATS' CENTRAL NERVOUS SYSTEM AFTER ELECTRICAL STIMULATION OF THE GREAT SPLANCHNIC NERVE

有关内脏痛的问题，已有不少学者进行了研究。但由于内脏痛本身的复杂性，至今其中枢传导途径还不太清楚。本实验刺激大鼠内脏大神经后，利用Ｆｏｓ的免疫组化技术在下列结构中可以发现Ｆｏｓ免疫组化阳性反应的神经元：隔外侧核、斜角带核、丘脑的菱形核、连接核、下丘脑室旁核、中缝背核、兰斑、中缝大核、网状外侧核、脊髓ＲｅｘｅｄⅠⅡⅢⅩ层等结构。而在对照组的上述结构中没有表达，只在耳蜗背核有较明显的表达。通过上述研究我们发现围绕脊髓中央管和各脑室的周围灰质系统与内脏伤害性刺激有关。这引起了我们极大的兴趣。关于这部分的工作我们正在进行中。

Although a lot of researches have been focused on visceral pain, up to now, the central pathway of visceral pain is still nuclear. In our paper, after stimulating the rats' great spanchnicnerve, the expression of Fos was examined by using Fos immunohistochemical technique.Obvious Fos expression was shown in the following structures: nucleus lateralis septi, nucleus tractus diagonalis, nucleus,paraventricularis (hypothalami), nucleus rapbe dorsalis,locus coeru lens, nucleus raphe moguus nucleus reticularis lateralis, laminae Ⅰ, Ⅱ, Ⅴ, Ⅹof the spinal cord. little expression was observed in the structures mintioned above. It issuggested that the paraventricularis structures from the central canal of the spinal cord to theventricularis system are related to visceral nociception. This leads us to further research.