实验性脾虚小鼠脾脏淋巴细胞增殖周期和免疫细胞化学的研究

STUDY ON CELL PROLIFERATING CYCLE AND IMMUNOCYTOCHEMISTRY OF LYMPHOCYTES IN MOUSE SPLEEN WITH EXPERIMENTAL SPLEEN DEFICIENCY SYNDROME

本文用流式细胞术（ＦＣＭ）和免疫细胞化学方法，检测了实验性小鼠脾的淋巴细胞增殖周期和５－溴脱氧尿苷阳性细胞（ＢｒｄＵ￣＋）在脾脏内的分布．以及脾小结中ＩｇＭ￣＋细胞的反应。结果显示由利血平诱发的脾虚小鼠Ｓ期细胞数最少（占５％），Ｇ＿１和Ｇ＿２＋Ｍ期细胞增加。经过健脾汤治疗后Ｓ期细胞增至１０％，与对照组比较无显著性差异．但高于自然恢复组（Ｐ＜０．００１）。经免疫细胞化学显示：脾虚组小鼠的脾中５－溴脱氧尿苷阳性细胞（ＢｒｄＵ￣＋）的数量远低于其它组。脾小结缩小，帽状区ＩｇＭ￣＋Ｂ细胞基本消失，经健脾汤治疗后，复健组的脾小结与自然恢复组比较，体积明显增大，生发中心的ＢｒｄＵ￣＋和帽状区ＩｇＭ￣＋Ｂ淋巴细胞均相应增多。提示健脾汤有促进ＤＮＡ和ＩｇＭ合成及细胞增殖作用。

The FCM and immunocytochemistry have been used to detect the cell proliferating cycle,the distribution of BrdU￣+cells in spleen and the reaction in splenic nodules in different groups of mice.The results indicated that the number of cells of S-phase was smallest loest(5%)in spleen deficiency group induced by reserpine,while their B cells of G1 and G2+M phase increased.After treatment with Jian Pi decoction,the cell number of S-phase increased to 10%.which showed no statistieal significance compared with the control group,but was significanty higher than that in the spontanous recovery group(P<0.001).Form the Immunocytochemical results we discovered that the number of BrdU￣+ cells in the spleen of spleen deficiency mice was much lower than in the others.In addition,the size of splenic nodules was reduced and IgM￣+cells on the mantle zone basically disappeared.After treatment with Jian Pi decoction,the splenic nodules were obviously increase compared with spontanous recovery group, which indicated that the numbers of both BrdU￣+cells in germinal center and the IgM￣+ cells on the mantle zone inereased corresponding These this findings suggest that the Jian Pi decoction has an effect promoting the synthesis of DNA and immuoglobulin and its cell proliferating.