不同冷冻条件对骨髓造血细胞的CFU－GM形成能力及DNA活性的影响

INFLUENCE OF DIFFERENT CRYOPRESERVATION METHODS ON ACTIVATION OF CFU-GM AND DNA OF HUMAN MARROW CELLS

本文对不同冷冻条件下人骨髓造血细胞的粒单系集落（ＣＦＵ－ＧＭ）形成能力及ＤＮＡ活性变化进行测定。结果证明：室温下低温保护剂二甲基亚砜（ＤＭＳＯ）使骨髓造血细胞形成ＣＦＵ－ＧＭ的能力下降；程序降温至－８０℃与降至－８０℃后与在液氮内冻存７天骨髓造血细胞相比较，二者形成ＣＦＵ－ＧＭ的能力以及ＤＮＡ活性均无明显变化；在程序降温过程中，消除融合热释放组骨髓造血细胞形成ＣＦＵ－ＧＭ的能力明显高于来消除融合热释放组。

Influence of different cryopreservation on the ability of CFU-GM formation and DNA activation of human marrow cells was studied. The results showed that with increasing duration of DMSO incubated with marrow hemopoitic cells at the temperature of 25℃,the amount of CFU-GM colony in culture medium was reduced.Activation of DNA and the ability of CFU-GM formation of marrow cells stored in liquid nitrogen were no striking difference from that of the cells stored at-80℃ or lower than-80℃ group.The ability of CFUGM formation in heat release group was remarkably higher than that in control one.