西藏地区沙门氏菌属的PCR检测及病原体耐药性研究

STUDY ON DETECTION OF SALMONELLAE WITH PCR AND THEIR DRUG-FAST IN TIBET

本文报告了以鼠伤寒沙门氏菌的鞭毛素基因(flic)序列的一对各21mer为引物,采用聚合酶链反应(PCR)技术检测西藏高原地区(平均海拔3600m以上)采集的626例急性腹泻患者粪便标本。结果检出沙门氏菌阳性11株。经生化和血清学分型鉴定,确定其中3株为B群鼠伤寒沙门氏菌(S.typhi—murium);8株为C群中沙门氏菌(S.boris—morbificans)。11株沙门氏菌与29种常用药物药敏实验结果显示;16种药物敏感;13种药物均有不同程度耐药。耐药率高达63.6—100％,11株沙门氏菌呈现4—10种多重耐药性。

The fecal samples of 626 acute diarrhea patients in Tibet had been examined with PCR technique. A pair of the gene sequence which contained 21 mer of the flagellin (file) were taken as the guide. 11 strains of the positive salmonellae were detected. After the biochemical and serumal identification, it was confirmed that 3 of 11 strains were s. typhi-muriun and 8 were S. bovis - morbificans. The results of the drug -fast sensitive experiments among the 11 strains and 29 kinds of the medicine showed that these salmonellae were sensitive to 16 drugs and drug -fast to other 13 ones. The drug -fast rate of the comman antibiotis, such as tetracycline, chloramphonicol, ploymyxin B, doxvcycline, streptomycin and sulfanilamide, reached to 63. 6 -100%. All of these bacteria were drug -fast to 4 - 10 kinds of the antibiotics.