HL－60细胞在维甲酸诱导过程中细胞动力学变化

Changes of Cell Cycle Kinetics of HL-60 Cell Induced by Retinoic Acid

用Ｂｒｄｕ／ＤＮＡ双参数流式细胞仪技术研究了ＨＬ－６０细胞经全反式维甲酸（ＡＴＲＡ）和９－顺式维甲酸（９－ｃｉｓＲＡ）诱导２４，４８和９６ｈ后，细胞周期动力学各参数，ＢｒｄＵＬＩ，Ｔｓ，Ｔｐｏｔ和ＰＲ的变化，同时测定细胞ＮＢＴ还原试验和细胞形态学的变化，发现随着维甲酸诱导时间的延长，ＢｒｄＵＬＩ逐渐降低，ＰＲ亦是逐渐减少，而Ｔｓ和Ｔｐｏｔ则逐渐延长。ＮＢＴ还原试验结果表明ＮＢＴ阳性细胞百分比呈现上升趋势：细胞形态学观察显示稍成熟阶段细胞百分比上升，早幼粒阶段细胞所占百分比下降。本文观察了ＡＴＲＡ和９－ｃｉｓＲＡ在诱导ＨＬ－６０细胞过程中以上各参数的变化，结果提示两类维甲酸对ＨＬ－６０细胞均有诱导分化功能。

Changes of cell cycle kinetics of HL-60 cell line after ALL-trans retinoic acid(ATRA)and 9-cis retinoic acid(9-cis RA)induction were studied with BrdU/DNA bivariated flow cytometry. HL-60 cells were exposed to ATRA or 9-cis RA for 96 h. During the exposure, the RA-treated cells were obtained at 24,48,72 and 96 h and then the cell cycle kinetics was meas ured with BrdU/DNA bivariated flow cytometry.Data from 4 parameters of cell cycle showed that treatment with ATRA or 9-cis RA for 96 h resulted in a decrease in the cell BrdU LI,production rate and an increase in Ts and Tpot. Mean while the ability of the cell to reduce NBT was enhanced by RA induction, and the percentage of matured cells was increased by cell morphology assessment.These results suggested that both ATRA and 9-cis-RA may induce HL-60 cell differentiation.