内皮素对血管平滑肌细胞中一氧化氮合成酶基因表达的影响

INF LUENCE OF ENDOTHELIN ON EXPRESSION OF NITRIC OXIDES YNTHASE (NOS)OFVSMCGENE

应用反转录－聚合酶链式反应（ｒｅｖｅｒｓｅｔｒａｎｓｃｒｉｐｔｉｏｎ－ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＲＴ－ＰＣＲ）方法，测定出一氧化氮合成酶（ＮｉｔｒｉｃＯｘｉｄｅＳｙｎｔｈａｓｅ，ＮＯＳ）ｍＲＮＡ不仅在心血管系统细胞中广泛存在，并且发现内皮素可以促进血管平滑肌细胞中ＮＯＳｍＲＮＡ的表达（＋３４．８％），增高血管平滑肌细胞中ＮＯＳ酶活性（＋７４％）。结果提示，由内皮素引起的血管平滑肌细胞中ＮＯＳ基因表达和ＮＯＳ活性的增加，可能是高血压中的一个重要的负反馈因素。

ith reverse transcription polymerase chain reaction(RT-PCR),it had been found that NOS mRNA is widely distributed in cells of cardiovascular system such as cardiocvtes,endothelial cells,VSMCs, When ET(10- mol/L) was added to cultured VSMC,it significantly increased the NOS mRNA level in VSMC( +34.8%) .By measuring the enzyrme activity of NOS in cultured VSMC,it is foumd that when VSMC was treated with ET(2xl0-7 mol/L,NOS activation is significantly increased(79%)compared to the control.These da-ta showed that the increased expression of NOS gene in VSMC induced by ET may be an important regulatory factor in hypertension.