ET和IL－1对VSMC中NOS基因表达的调控及其生物学意义

REGULATION AND BIOLOGICAL EFFECTS OF ENDOTHELIN AND INTERLEULIN- 1 ON EXPRESSION OF NI TRIC OXIDE SYNTHASE GENE I N VAXCULAR SMOOTH MUSCLE CELLS

应用反转录－聚合酶链式反应（ＲＴ－ＰＣＲ）和３Ｈ－胸腺嘧啶核苷参入技术发现，在正常生理状态下，内皮素可与大鼠血管平滑肌细胞（ＶＳＭＣ）上内皮素Ａ型受体、内皮素Ｂ型受体结合，通过调控一氧化氮合成酶（ＮＯＳ）ｍＲＮＡ转录表达水平，从而调节ＶＳＭＣ的分裂和增殖。白细胞介素－１对ＶＳＭＣ中ＮＯＳ基因表达的促进作用和对ＶＳＭＣ分裂的影响，可能部分是通过内皮素及其受体这一途径完成的。

By regulation the expression of nitric oxide synthase(NOS) mRNA,endothelin binded ETA - Rand ETB - Ron the vascular smooth muscle cells(VSMC )of rat affects the mitogenesis and proliferation of VSMCwith reverse transcription- polymerase chain reaction(RT- PCR)and3H- thymidine incorporation.Interleulin-l enhances the expression of NOS gene and affects the mitogenesis of VSMC partly through the pathway ofendothelin and itsreceptors.