动脉粥样硬化斑块组织中几种基因的表达（免疫组织化学和原位杂交）

EXPRESSION OF SEVERAL GENES IN ATHEROSCLEROTIC PLAQUE BY IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION

应用免疫组织化学和原位杂交方法，检测人和家兔实验性动脉粥样硬化（ＡＳ）时内皮素（ＥＴ）及其Ａ型受体、血管紧张素Ｈ一Ａ型受体（ＡⅡＡ－Ｒ）、一氧化氮合成酶（ＮＯＳ）和ｐ５３抑癌基因表达的变化，发现在ＡＳ斑块中ＥＴ及其Ａ型受体、ＡⅡＡ－Ｒ基因表达增加，ＮＯＳ基因表达减少，并有突变型ｐ５３基因表达增加。提示ＥＴ和ＡⅡ以及ＮＯＳ基因表达的改变可能参与ＡＳ时血管平滑肌细胞增殖过程；ｐ５３抑癌基因突变也可能与之有关。

resent work observated gene--expressing alteration of endothelin(ET)and its type A receptor(ETA--R),Type l receptor of angiotensin II(AT1 --R),nitric oxide synthesase(NOS)as well as the expressing al-teration of anti oncogene p53 in human s and rabbit s atherosclerotic plaque using the method of immunohis-tochemistry and in situ hubridization. It was found that gene expression of ET,ETA--R,AT1--R is in-creased while the gene expression of NOS is reduced. Else,the gene expression of mutating type of p53 is in-creased.It was suggested that gene--expressing alteration of ET,AII and NOS may play a important role in the proliferation caurse of vascular smooth muscle cells in atherosclerosis and mutation anti--oncogene p53maybe is related to it too.