The cloning of Na+/Ca2+exchaanger Gene and its expression in brain ischemia

THE CLONING OF Na+/Ca2+ EXCHANGER GENE AND ITS EXPRESSION IN BRAIN ISCHEMIA

ＴＨＥＣＬＯＮＩＮＧＯＦＮａ￣＋／Ｃａ￣（２＋）ＥＸＣＨＡＮＧＥＲＧＥＮＥＡＮＤＩＴＳＥＸＰＲＥＳＳＩＯＮＩＮＢＲＡＩＮＩＳＣＨＥＭＩＡＴａｎｇＪｉａｎ汤健，ＷａｎｇＹｕ王瑜，ＺｋａｎｇＣｈｅｎｈｕｉ张晨晖，Ｅ．ＣｏｓｔａＩｎｓｔｉｔｕｔｅｏｆＣａｒ...

It is known that intracellular Ca2+ is overloaded during ischemia,and at the same tirne,the activity of one of the major pathways of ca2+ efluxs-Na+/Ca2+ exchanger is altered.However,the molecular mechanism of this alteration is still unknown. We cloned a 456bp cDNA fragment of rat cerebellar Na+/Ca2+ exchanger gene.Using this fragment as the probe,We found that the Na+ /Ca2+ exchanger mRNA is widely distributed in rat central and peripheral nervous system,such as in cerebra, heart,lung, and kidney. Using focal cerebral ischemic model,We de-tected the gene expression of Na+/Ca2+ exchanger in ischemic brain by Northern blotting and in situ hybridization,and found that in ischemic tissues,the mRNA level of Na+/Ca2+ exchanger is lowered.