制备性SDS—PAGE纯化大肠杆菌表达的重组人VEGF

ISOLATON AND BIOACTIVITY REECOMBINANT HUMAN VEGF FROM THE PROCAARYOTIC EEXPRESSION PRODUCTS IN E.C0LI

在以基因工程方法实现大肠杆菌表达外源重组人ＶＥＧＦ的基础上，应用制备性ＳＤＳ—ＰＡＧＥ从包含体中分离纯化了这一因子。纯化蛋白在含有氧化型与还原型谷胱甘肽和肝素的缓冲液中复性，复性后的ＶＥＧＦ蛋白成为同源双体，经Ｍｉｌｅｓ方法检测，证明可提高血管通透性，具有生物活性。这一研究为制备具有生理与病理意义的研究奠定了基础。

n the basis of expressing recombinant human VEGF in E. Coli we reported earlier,we report the isola-tion and bioactivity recovery of this factor from the procaryotic exprexsion products in this studyIsolated re-combinant human VEGF is renatured in buffer containing oxidized and reduced glutathione forms homodimer shown in non-reduced analytical SDS-PAGE. Miles assay shows the renatured VEGF promotes vessel perme-ability in dose-petency manner.