心血管疾病基因治疗的基础研究Ⅵ．一种新的体内基因转移方法

BASIC RESEARCH OF GENE THERAPY ON CARDIOVASCULAR DISEASE Ⅵ.A NEW METHOD FOR GENE TRANSFER IN VIVO

将医用缝合线浸泡在质粒ＤＮＡ溶液中，然后缝合在大鼠股四头肌上，可以观察到外源基因在肌肉组织中高效、稳定地表达，其表达量高于磷酸钙共沉淀和ＤＮＡ沉淀块埋植法，较肌肉直接注射法表达量高１０倍左右。

new method was found to improve the efficiency of direct gene transfer in skeletal muscle.pN2-LacZ plasmid DNA was dissolved in PBS containing 5%sucrose. Medical suture was soaked in the plasmid solution for 24 hours before stitched on the quadricep muscle,The same amount of DNA was transfered by calcium phosphate coprecipitation implantation,DNA precipitation implantation or direct injection into the quadricep muscle. LacZ gene expression was detected by β-galactosidase assay and histochemical staining,and the effi-ciency of foreign gene expression by stitching was much higher than the other methods mentioned above.