BQ＿（123）－多聚赖氨酸携载β－半乳糖苷酶基因向血管平滑肌细胞的靶向导入

BQ123-POLYLYSINE MEDIATED INTRODUCTION OF pSV-β-LacZ VASCULAR SMOOTH MUSCLE CELLS IN VITRO

本研究连接合成了ＢＱ１２３－多聚赖氨酸（ＢＰ）利用ＢＱ１２３对血管平滑肌细胞（ＶＳＭＣ）膜表面内皮素Ａ型受体（ＥＴＡ）的高度选择性和多聚赖氨酸对ＤＮＡ的强吸附作用，以ＢＰ携载含有β－半乳糖苷酶（β－ＬａｃＺ）基因的表达质粒ｐＳＶ－β－Ｇａｌａｃｔｏｓｉｄａｓｅ（ｐＳＶ－β－ＬａｃＺ）对培养的ＶＳＭＣ和内皮细胞（ＥＣ）转移基因。结果表明，ＢＰ具有向ＶＳＭＣ转基因性能，且依赖与靶细胞表面ＥＴＡ的结合。

We constructed a soluble targeted DNA delivery carrier,BQ123-Polylysine,by which plasmid DNA (pSV-β -LacZ)was transfered into vascular smooth muscle cells(VSMC).The expression levels of the re-porter gene(β-LacZ)in treated VSMC and endothelial cells show that BQ123-Polylysine can efficiently trans-fer DNA into VSMC,mediated by endothelin A subtype receptors of VSMC surface.The results suggested that it is possible for investigators to use this delivery carrier as a tool in research VSMC gene regulation and cardiovascular gene therapy.