山莨菪碱治疗环孢素肝毒性的机理及临床应用研究

The Effect of Anisodamine on the CsA Hepatotoxicity and Clinical Study

SD大鼠分4组，即对照组、环孢素（CsA）组、CsA+小剂量山莨菪碱组、CsA+大剂量山莨菪碱组。用药前、用药1周及2周分别检测血甘氨胆酸、胆红素、白蛋白、丙氨酸转氨酶、天冬氨酸转氨酶及碱性磷酸酶。用药2周后检测肝组织甘油三酯、脂质过氧化产物（LPO）、超氧化物歧化酶、还原性谷胱甘肽（GSH）及细胞色素P450（P450）。结果表明山莨菪碱可减轻CsA引起血甘氨胆酸、胆红素、白蛋白，肝匀浆LPO、GSH及P_(450)的异常变化。49例CsA肝损害的肾移植受者经山莨菪碱治疗，其中35例（71.4％）治愈，6例（12.2％）好转，无效8例（16.3％）。

SD rats were divided into 4 groups:control,CsA,CsA+anisodamine(5mg／kg·d)and CsA+anisodamine(20mg／kg·d)groups.The serum glycocholic acid(GCA),bilirubin(Bil),albumin,ALT,AST,ALP were measured before and 1,2 weeks after the administration.The triglyceride(Trig),LPO,GSH,SOD,and P450 of liver homogenate were assayed 2 weeks after the beginning of the administration.The results showed that anisodamine could attenuate the abnormalities in serum GCA,bilirubin,albumin,liver LPO,GSH and P450.49 patients with liver damage caused by CsA hepatoxicity were treated with anisodamine,The results showed that 35 cases(71.4％)were cured with SGPT and serum bilirubin returning to normal,6 cases(12.2％)were improved and 8 cases failed to respond to the therapy(16.3％).