柞蚕抗菌肽D基因转化广藿香的研究

Transfer of Cecropin-D Gene into Pogostemon Cablin

结果表明，柞蚕抗菌肽Ｄ（Ｃｅｃｒｏｐｉｎ－Ｄ）对广藿香青枯病的病原细菌ＰｓｅｕｄｏｍｏｎａｓＳｏｌａｎａｃｅｒｕｍ有很强的抑制作用。通过农杆菌介导的方法将Ｃｅｃｒｏｐｉｎ－Ｄ基因导入广蕾香细胞，获得５株转基因植株，并繁殖成无性系。５个无性系在与病原菌共培养时都表现出一定的抗性。

Bacterial wilt is a severe disease on Pogostemon cablin in Hainan. Our tudy showed that Cecropin-D had significant inhibitory effect on the pat-hogen of the disease,Pseudomonas solanaserum. Successful transfer of Cecr-opin D gene into the cells of Pogostemon cablin were made through Agroba-cterium-mediating method. Five transgenic plants have been obtained and cloned. The clones of the transgenic plants displayed certain resistance to the pathogen when they were co-cultured with the latter.