LARGE　SCALE　PURIFICATION　OF　PHOSPHOGLYCERATE　KINASE（PGK）　AND　GLYCERALDEHYDE　3－PHOSPHATE　DEHYDROGENASE（GAPDH）　FROMYELLOW　PEAS　BY　PEG／REPPAL　PES　AQUEOUS　TWO　PHASE　SYSTEM　AND　ION　EXCHANGE　CHROMATOGRAPHY

ＬＡＲＧＥＳＣＡＬＥＰＵＲＩＦＩＣＡＴＩＯＮＯＦＰＨＯＳＰＨＯＧＬＹＣＥＲＡＴＥＫＩＮＡＳＥ（ＰＧＫ）ＡＮＤＧＬＹＣＥＲＡＬＤＥＨＹＤＥ３－ＰＨＯＳＰＨＡＴＥＤＥＨＹＤＲＯＧＥＮＡＳＥ（ＧＡＰＤＨ）ＦＲＯＭＹＥＬＬＯＷＰＥＡＳＢＹＰＥＧ／ＲＥＰＰＡＬ...

Abstract A process to purify phosphoglycerate kinase(PGK) and glyceraldehyde 3-phosphate dehydrogenase(GAPDH) from yellow peas by PEG/Reppal PES system and ion exchange chromatography in large scale was developed. The recovery and purification factor of PGK were 41% and 41, and that of GAPDH were 57% and 113,respectively.