常规三维荧光光谱的数学模拟方法研究

Studies on Three-Demensional Fluorescence Specta with Mathematic Simulation Method

采用Ｆ分布的数学模式，对盐酸硫胺素（Ｖ＿（Ｂ＿１））和盐酸吡多辛（Ｖ＿（Ｂ＿６））的荧光激发和发射光谱分别进行数学模拟，得到四个数学方程，根据荧光激发光谱与发射光谱间所具有的线性相交特性和荧光相对强度所具有的加和性，可分别求得两物质单、双组份体系波长型三维荧光光谱的数学方程。比较数学模拟和实测得到的单、双组份体系波长型三维荧光光谱，两者基本一致。将波长型三维荧光光谱数学方程中的波长变量转换成能量变量，就得到两物质的各种能量型三维荧光光谱数学方程和谱图。

Addopting F distribution, four mathematic models of fluorescence exitation andemission spectra of vitamin B_1 and vitamin B_6 were achieved with mathematic simulated method,Themodels were in keeping with the experimental data of excitation and emission spectrum, According tothe characteristic of linear intersection between excitation and emission spectrum,and of additive inrelative fluorescence intensity, the equations of EEM of single or two components system were ob-tained.