铅对仔鼠行为功能影响的实验研究(一)──雌性小鼠接触铅对仔鼠行为功能的影响

Study on the effect of behavioral function by ph (1)

我们以１０００ｍｇ／Ｌ、５００ｍｇ／Ｌ、５０ｍｇ／Ｌ三个剂量组对雌性昆明种小鼠进行醋酸铅自由饮用染毒。染毒时间分两种方式：Ａ为染毒至交配；Ｂ为染毒至产仔。通过交叉抚养程序，观察其仔代的行为功能改变，并对仔鼠的中枢神经系统做了病理组织学检查。结果发现：１、雌性小鼠染毒各剂量组胎鼠形态未见改变，而仔鼠行为功能发生异常，表明行为改变是反应发育过程中神经系统受损的较为敏感的指标。２、雌性小鼠接触铅后，其血铅水平在远低于我国铅中毒诊断标准（５０ ｇ／ｄ１）情况下，其仔代的行为功能就发生异常，提示铅由胎盘和乳汁传递给胎儿，并影响仔代的中枢神经发育。３、电镜超微结构显示仔鼠中枢神经系统（皮质、内囊、丘脑）的中毒性损伤，是动物行为异常的病理基础。

The lead acetate were dissolved in drinking water administrated to female kuen--Mingmice ad fib at 1000, 500 & 50mg/L dose levels. The duration of administration were in twoforms: A-from begining of administration to mating, B--from begining of administration toparturition and following cross feeding schedule' The behavioral functions of the offspringswere observed' The histology and pathology of centrol nerve system of the progeny were examined. The results showed: 1. No morphological changes were found in the litters of thetreated female mice, but abnormal behavioral function do exist, which indicated that the behavioral function is a sensitive index for the damage occured in developing centrol nerve system. 2. After lead expoure even though the blood lead level of the female mice, lower than50ig/dl (intoxication criterian ), the behavioral function of the progeny turned abnormal'The influence of group A was more evident' This result showed that lead could be transported to fetues through placenta and milk inducing adverse effect on centrol nerve system.3. The electronic microscopic examination confirmed, the damages, in centrol nerve systemsthat may be the pathological basic of behavioral changes.