再生障碍性贫血外周血单核细胞中肿瘤坏死因子基因表达的研究

^^The Study for the Gene Expression of a -TNF in Peripheral Blood Mononuclear Cells from Patients with Aplastic Anemia

本文报道了用Ｎｏｒｔｈｅｍｂｌｏｔ方法检测再障患者、正常人ＰＢＭＣ和经删Ｒａｄ６０Ｃｏ－ｙ全身照射、正常对照小鼠脾细胞的ＴＮＦｍＲＮＡ的表达。结果显示：７例再障患者中有３例测不到ＴＮＦｍＲＮＡ，２例含量极低（共占７０．４％）；而正常对照８例中除１例外其余均较高（占１２．５％）。在小鼠中照射组１０例除１例外其余均明显降低（占９０％）；对照组仅１例降低（占１０％）．结果提示再障时ＰＢＭＣ中的ＴＮＦｍＲＮＡ表达是降低的（Ｐ＜０．０１）。

The gene expression of a-TNF in PBMC(peripheral blood mononuclearcells)from patients with aplastic anemia and in the spleen cells from BABL/C mice treated with 600 Rad60 Co-r nomoral controls was examined using Northem Blot and compared withnormal controls The results showed that a-TNF mRNA of PBMC in 32 of 7 aplastic anemia patients were undetectable ,and 2 of them expressed extremely low level from eight nor-mal individuals Moreover lower lower levels of the gene expression were detected in 9of 10 of the tre-ated Bablc mice (90%),compared with only 1 in 10 of normal controls (10) the results sug-gest that gene expression of a- tNF was lower in PBMC from patients with aplastic anemia(P<0.001).