簇毛麦（Haynaldia Villosa）的原生质体培养

PROTOPLAST CULTURE OF HAYNALDIA VILLOSA

簇毛麦的幼胚在含有９μｍｏｌ／Ｌ２，４－Ｄ的ＭＢ固体培养基上诱导产生愈伤组织，在相同的培养基上继代培养两个月后出现淡黄色的致密瘤状愈伤组织．在继代培养过程中，愈伤组织的分化能力丧失较快．继代培养一年左右，改变培养条件，可得到颗粒状及粉粒状愈伤组织．分别用这两种类型的愈伤组织建立悬浮培养细胞系．愈伤组织的形态结构与悬浮系的建立及原生质体培养密切相关．来源于颗粒悬浮系的细胞团块产生的原生质体分裂后可产生体细胞胚，而用粉粒悬浮系分离的原生质体不能持续分裂．

White and soft calli were induced from immature embryos of Haynaldia villosa on MBsolid media containing 2,4-D(9μmol/L).After two months of subculture,they turned to belight yellow and compact,and about one year later,two kinds of callus(grainy and powdery)were formed when the 2,4-D concentration was raised to 18～36μmol/L. These two kindsof callus were used to establish cell suspension. The nature of the callus used was a criticalfactor in establishing cell suspensions and isolating protoplasts. Protoplasts derived fromgrainy suspension divided and formed embryoids,but sustained division were not observed inthe culture of protoplasts derived from powdery suspension.