紫外线照射引起血管内皮细胞损伤及其对TM活性的影响

Study on the Vascular Endotheliocyte Injury and the Changes of its TM Activity by Irradiation with Ultroviolet Rays

本研究以紫外线作为损伤因素,并加以定量,以观察紫外线照射对血管内皮细胞的影响。结果表明,在不同程度的紫外线作用下,血管内皮细胞出现变园浮起、细胞溶解的不同的形态变化特征,当照射量大于1J/cm^2时,其存活率显著降低(P<0.01)。同时,在紫外线照射作用下,内皮细胞表面的血栓调节蛋白(Thrombomodulin简称TM)活性降低,其降低程度与照射量及照射后经过的时间有关。0.1J/cm^2照射量组24小时后可见明显下降(P<0.05);1J/cm^2照射量组3小时后显著降低(P<0.01);13.3J/cm^2照射量组照射后立即测定TM活性.可见显著降低(P<0.01),且随着时间的增加呈持续下降趋势。

The influence of irradiation with quantitive ultroviolet rays on tne vascular endothelio-cyte were investigated in this paper. The result indicated that the morphological changes of endothe-liocyte such as rounding up, floating and solving in the medium were varied after irradiation with different amounts of ultrovolet rays. The rate of endotheliocyte existance was also affected in varying degrees. Further, the TM activity on the surface of endotheliocyte were significately dereased when the amounts of irradiation were increased.