摘要
In the present study we have found that proto-oncogene c-fos protein can expressin the noradrenergic neurons of rat hindbrain following peripheral electrical stimulation. Ratswere given peripheral electrical stimulation via thin stainless steel pins inserted into the pointsnear knee joint (S36) and ankle joint (Sp6) which mimic the manipulation of electroacupuncture(EA) performed in humans. Animals were perfused for double staining immunohistochemistry 2hafter the termination of EA. In rats subjected to EA stimulation Fos-like protein was found in thetyrosine hydroxylase (TH)-like immunoreactive neurons in rat hindbrain. The Fos and TH coex-isting neurons were distributed in the locus coeruleus, solitary tract nucleus, ventrolateral medul-la, periaqeductal gray, as well as superior colliculus. The percentage of the coexisting neuronscompared with the total number of neurons containing Fos-like protein in these nuclei rangedfrom 6% to 32%. The results suggest that the noradrenergic neurons in these regions may be ac-tivted by acupuncture stimulation.
In the present study we have found that proto-oncogene c-fos protein can express in the noradrenergic neurons of rat hindbrain following peripheral electrical stimulation. Rats were given peripheral electrical stimulation via thin stainless steel pins inserted into the points near knee joint (S36) and ankle joint (Sp6) which mimic the manipulation of electroacupuncture (EA) performed in humans. Animals were perfused for double staining immunohistochemistry 2h after the termination of EA. In rats subjected to EA stimulation Fos-like protein was found in the tyrosine hydroxylase (TH)-like immunoreactive neurons in rat hindbrain. The Fos and TH coex- isting neurons were distributed in the locus coeruleus, solitary tract nucleus, ventrolateral medul- la, periaqeductal gray, as well as superior colliculus. The percentage of the coexisting neurons compared with the total number of neurons containing Fos-like protein in these nuclei ranged from 6% to 32%. The results suggest that the noradrenergic neurons in these regions may be ac- tivted by acupuncture stimulation.
基金
Chinese postdoctoral foundation
a grand from the National Institute of Drug Abuse, USA (DA 03983)
