感染日本血吸虫病兔循环抗原的提纯与分析

Purification and Analysis of the Circulating Antigens of Schistosomiasis Japonica in Rabbits

采用免疫亲和层析法,从感染日本血吸虫的兔血清中提纯日本血吸虫病循环抗原。经SDS-聚丙烯酰胺凝胶电泳、PAS染色、酶联免疫印渍等方法分析,日本血吸虫病循环抗原显示7个组份,分子量分别为144、118、100、88、63、57、46kD;其中144、118、100、63、57kD为糖蛋白,并证实虫卵释放的循环抗原中含分子量分别为144、118kD的糖蛋白。

The circulating anligen (CAg) of schistosomiasis Japonica from the sera of Schi-stosoma japonicum-infected rabbits was purified by immune-affinity chromatography.The purified CAg exhibited seven protein bands by SDS-PAGE. The molecular weightsof them are 144, 118, 100, 88, 63, 57 and 46 kD respectively. These CAgs showedfive sugar protein bands by PAS colouring. The molecular weights of these sugarproteins are 144, 118, 100, 63 and 57 kD respectively. The CAgs released from theova of the schistosome contain 114 and 118 kD sugar proteins as shown by ELIB.