近交系小鼠超数排卵的研究

Study on Superovulation in Inbred Strain Mice

近交系小鼠超数排卵(简称超排)的研究可为小鼠胚胎库的建立提供大量胚胎。以孕马血清促性腺激素(PMSG)、人绒毛膜促性腺激素(HCG)为主,按不同品系小鼠的特点,加前列腺素(PGF_(2α))1μg/只以上。超排BALB/c系小鼠,平均每只雌鼠取可用胚胎22枚,C 57 BL/6 A系小鼠为23枚,对照组分别取得7枚和8枚,实验组与对照组差异显著(P<0.05)。超排处理后的雌鼠交配受孕率较高。实验结果提示改进的超排方法可提高国产激素制品的超排效果。

Superovulation in inbred mice can provide a lot of embryos for the establish-ment of mouse embryo cryobank. Pregnant mare serum gonadotropin and human cho-rionic gonadotropin were used for the superovulation in BALB/c and C 57 BL/6 Amice, satisfactory results were obtained by the addition of more than 1 μg of 15-methyl prostaglandin F_(2α) according to the characteristics of the mouse strains. Theresults show that the average usable embryos obtained from one superovulated BALB/cand C57BL/6 A mouse are 22 and 23 respectively, but only 7 and 8 in controlanimals. The pregnancy rate is higher in the treated mice than the controls.