摘要
Protoplasts isolated from immature cotyledons of cultivated soybean (Glycine max L.) were cultured by Gelrite bead method in ZSP medium with soybean root nodule products (asparagine, glutamine, allantoin, allantoic acid, etc.) as the main nitrogen resources. Embryogenic calli were initiated and transferred to somatic embryo differentiation medium. Somatic embryos were formed from embryogenic calli and converted to plants. The regenerated plants normally grew and flowered.
Protoplasts isolated from immature cotyledons of cultivated soybean (Glycine max L.) were cultured by Gelrite bead method in ZSP medium with soybean root nodule products (asparagine, glutamine, allantoin, allantoic acid, etc.) as the main nitrogen resources. Embryogenic calli were initiated and transferred to somatic embryo differentiation medium. Somatic embryos were formed from embryogenic calli and converted to plants. The regenerated plants normally grew and flowered.
