肝硬化、慢性乙型肝炎患者外周血自然杀伤细胞亚群的研究

A STUDY ON THE SUBSETS OF NATURAL KILLER CELLS IN THE PERIPHERAL BLOOD OF PATIENTS WITH LIVER CIRRHOSIS AND CHRONIC HEPATITIS B

用荧光激活细胞分类计数器检测了肝硬化和慢性乙型肝炎(慢乙肝)患者外周血中自然杀伤细胞(NK)的亚群状况。结果发现,肝硬化组的总 NK 百分比显著高于对照组和慢乙肝组(P<0. 04和 P<0. 005) ;慢乙肝组的 CD16^+CD57^-亚群百分比与细胞绝对数均明显低于对照组(P<0. 005) ;而肝硬化组的 CD16^+CD57^+亚群百分比则明显地高于慢乙肝组(P<0. 02) 。这些结果表明,慢乙肝患者体内的 NK 反应性低下,而肝硬化患者的 NK 反应性偏强。

Subsets of natural killer(NK)cells were determined in the peripheral blood of patients with liver cirrhosis(LC)and chronic hepatitis B(CHB)by fluorescence-activated cell sorter.The results showed that the percentage of the total NK cells in LC was significantly higher than those of the healthy controls and CHB(P<0. 04 and P<0. 005,respectively);the percentage and absolute number of CD16+CD57-cells in CHB were deceased as compared with the controls (P<0. 005) ;and the percentage of CD16^+CD57^+ cells in LC was significantly higher than those of the controls and CHB(P<0. 02) .Our results suggested that NK reactivity was lower in patients with CHB,and higher in LC patients.The clinical significance of these changes in the NK subsets was discussed.