血清胆碱酯酶同工酶对肝硬化的诊断价值

THE SIGNIFICANCE OF SERUM CHOLINESTERASE ISOENZYME IN THE DIAGNOSIS OF LIVER CIRRHOSIS

用管状聚丙烯酰胺梯度凝胶电泳法检测42例正常人及176例肝胆病患者的血清胆碱酯酶(SChE)同工酶谱,同时采用 Ellman 法测定 SChE 活性。结果表明正常人SChE 可分出8～12条同工酶带,绝大多数慢性肝病患者带6(ChE_6) 的活性升高。而带2～5(ChE_(2～5) )的活性降低。血清 ChE_6/ChE_2～CChE_5比值随肝病的加重而逐渐升高,尤以肝硬化时更为明显。血清 ChE_6/ChE_2～ChE_5比值升高对肝硬化诊断的阳性率明显高于A、A/G 和 SChE 活性。提示血清 ChE_6/ChE_2～ChE_5比值可作为一个诊断肝硬化新的血清酶学指标。

The isoenzyme patterns and the activities of serum cholinesterase(SChE)in 42 healthy adults and 176 patients with hepatobiliary diseases were studied by polyacrylamide gradient electrophoresis and Ellman's method respectively.8～12 SChE bands were demonstrated in the healthy adults.The relative increased activity of band 6 and decreased activities of bands 2,3,4and 5 were observed in most of the patients with chronic liver diseases as compared with the controls.The increased serum ChE_6/ChE_2～ChE_5 ratio correlated with the severity of the liver diseases,and its positive detection rate for cirrhosis was much higher than that of serum albumin,A/G and SChE activity.Our results suggest that serum ChE_6/ChE_2～ChE_5 ratio is a useful and sensitive enzymic marker for the diagnosis of liver cirrhosis and for judging the severity of liver diseases.