红树植物叶片叶绿素提取方法比较及其活体测定

Comparision of Extraction Methods Used to Determine Chlorophyl Content in Mangrove Leaves and Alive Leaf Determination

本文采用混合液法测定了五种红树植物叶片的叶绿素含量,并摸索了提取过程中混合液的不同配比和提取的时间。对相同的材料用 Arnon 法和混合液法进行对照测定,结果表明 Arnon 法比混合液法提取红树植物叶片叶绿素效果好.通过 Arnon 法和活体叶绿素仪的平行测定,分别建立了四种红树植物叶片的活体测定值 D 与叶绿素含量(mg/cm^2)的回归方程,由此得出快速测定红树植物叶片叶绿素含量的简便方法。

This paper deals with the methods of extracting chlorophyl from mangrove leaves.We apply mixed-solution method to extract chlorophyl of five mangrove species from Dandou and Daguansha,Beihai,and grope for varied solution proportions and extracting time during the extraction process.In comparing the mixed-solution method with Arnon method,We abtain a conclusion that Arnon method is more fit for chlorophyl extraction from mangrove plants.From parallel determination of Arnon method and HYL-1 chlorophyl instrument,the four mangrove species regression equations about chlorophyl content (mg/cm^2)and value D are given out.