用氧暴露时 PS 系统的变化对 UPTD 计量进行定量分析

Analysis of UPTD with the Changes of Pulmnary Surfactant System Caused by Oxygen Exposure in Rats

本研究应用电镜组化和膜天平测量法,对96只 Wistar 大鼠氧暴露400、615和814UPTD 后肺表面活性物质(Pulmonary Gurfactant,PS)系统变化进行了动态观察。结果发现连续氧暴露达615UPTD 时,即可见PS 分布不均,断裂和脱落、甚至出现局灶性肺泡萎陷、肺间质中性粒细胞浸润,PS 最小表面张力升高。连续氧暴露814UPTD 时,可见少数肺泡Ⅱ型上皮细胞变性,坏死,PS 最小表面张力随暴露剂量增加进一步升高。等剂量的间断暴露,各种反应明显减轻,PS 最小表面张力上升斜率明显小于连续氧暴露。提示:间断氧暴露与等剂量的连续暴露比,间断在氧对肺组织的损伤发生、发展中可能具有重要意义,从而为氧的合理利用提供了实验证据。

96 wistar rats were divided into 5 groups at random,i,e,400,615,814 UPTD oxygen exposure,normobaric oxygennitrogen exposure and control.There were two manners of oxygen exposure:continuous exposure or intermittent exposure.The changes of pul- monary surfactant system(PS)were observed in all animals after hyperbaric exposures.It was found that under the condition of continuous oxygen exposure at 615 UPTD,the PS lay- er on some of aveoli became discontinued,aggregated or broken away from the aveoli.sur- face,and atelectasis occured in some of the aveoli.Under the condition of continuous oxygen exposure at 814 UPTD,a few of type Ⅱ alveolar cells necrotized or disrupted,minimal sur- face tension of PS increased and neutrophil infiltrated into the alueolar septa.On the con- trary,the changes observed under the intermittent oxygen exposure were much more unre- markable comparing to those observed under the continuous oxygen exposure.This result in- dicated that intermittence in the oxygen exposure may have an important role in the develop- ment of pulmonary oxygen poisoning and suggested to us that intermittent oxygen exposure may be a useful method in preventing pulmonary oxygen poisoning.