水解ATP制备环状腺苷酸的研究

Hydrolytic ATP for preparation of Adenosine-3':5'-cyclic Monophosphate (eAMP)

用0.3mol Ba(OH)_2 96℃加热40min水解ATP制得cAMP,纸电泳分离样品中的cAMP,紫外吸收测定其含量。结果表明,ATP水解后cAMP的生成率为85.3％,cAMP含量为10.6mg/ml。

Hydrolytic ATP used 0.3mol barium hydroxideat 96℃ for 40min, yielde cAMP The cAMP of sample was seperated by paper electropnoresis,its con tent tested by ultravioletabsorption。The results show that cAMP producting rate is 85.3 percent, and the content of cAMP is 10.6mg per ml.