快速Dot-ELISA诊断日本血吸虫病的初步研究

PRELIMINARY STUDY ON DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA BY FAST DOT-ELISA

本研究对Dot-ELISA进行了改进,建立了快速Dot-ELISA。运用该法检测了96份血吸虫病人血清,其中88份为阳性(91.7％),另检测了20份华枝睾吸虫病人及30份正常人血清作为对照,各有1份为阳性。与Dot-ELISA比较,两者的敏感性及特异性相似,然而快速Dot-ELISA具有快速(整个反应可在0.5h内完成)、简便、试剂用量少等特点,尤其在基层具有一定的推广应用价值。

Fast dot-ELISA (FD-ELISA) was established on the basis of dot-ELISA for the diagnosis of schistosomiasis japonica. 96 sera speimens from patients with schistosomiasis, 88 (91. 7%) were positive by this method: while with 20 sera specimens from patients with clonorchiasis sinensis and 30 sera specimens from healthy indivduals, only 1 specimen was positive respectively. The result showed that the specificty and sensitivity of FD-ELISA were similar to those of dot-ELISA, but this method had the advantage of being fast (taking-less than half hour), simple and saving reagents. It showed to be very suitable for field application.