ELISA检测蛔虫感染者血清抗体的观察

ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETECTION OF ANTI-ASCARIS ANTIBODIES IN THE RESIDENTS INFECTED WITH ASCARIS LUMBRICOIDES

采用ELISA检测蛔虫病流行区血清259例,其中193例粪检蛔虫卵阳性者,血清抗体的阳性符合率为93.8％:112例健康胎儿血清的阴性符合率为96.4％;56例肠道蠕虫卵阴性者血清的阴性率为82.1％;10例蛔虫卵阴性而肠道其它线虫卵阳性者血清的阳性率达30％。结果表明,此法诊断蛔虫感染具有较高的敏感性,但与肠道其它线虫感染血清有一定交叉反应。

259 sera from residants in an area epidemic for Ascaris were detected by ELISA. 93. 8% of 193 cases with ascaris eggs were positive while 108(96.4%) of 112 normal feotus sera were negative. The negative rate of 56 sera from cases confirmed negative for intestinal nema-tode eggs was 82. 2% and the positive rate of 10 sera from those negative for ascaris eggs but positive for other intestinal nematode eggs was 30%. The results showed that the sensitivity of ELISA for detection of anti-ascaris antibodies was high and there was some cross reaction with other intestinal nematodes.