摘要
A hepatitis C virus(HCV)cDNA fragment,534bp in length and designated asQ534,was obtained by PCR amplification with self-designed primers.Q534 was cloned in-to Hinc Ⅱ site of pUC18 and the recombinant plasmid pQ534 was then selected from thebacterial transformants.The sequence analysis indicated that Q534 was a cDNA fragmentof HCV core gene,and located in HCV genome from positions 320 to 853 incorrespondence with Chiron’s prototype sequence.The homologies between Q534 and theprototype at the levels of nucleotides and amino acids were 90.0% and 97.6%,respectively.The homologies of Q534 with Japanese HCV-J and HCV-BK strains were 96.6% and97.0% at the nucleotide level,and 98.2% and 98.8% at the amino acid level.In terms ofthe sequence,this Chinese HCV isolate should belong to HCV group Ⅱ.
A hepatitis C virus(HCV)cDNA fragment,534bp in length and designated as Q534,was obtained by PCR amplification with self-designed primers.Q534 was cloned in- to Hinc Ⅱ site of pUC18 and the recombinant plasmid pQ534 was then selected from the bacterial transformants.The sequence analysis indicated that Q534 was a cDNA fragment of HCV core gene,and located in HCV genome from positions 320 to 853 in correspondence with Chiron's prototype sequence.The homologies between Q534 and the prototype at the levels of nucleotides and amino acids were 90.0% and 97.6%,respectively. The homologies of Q534 with Japanese HCV-J and HCV-BK strains were 96.6% and 97.0% at the nucleotide level,and 98.2% and 98.8% at the amino acid level.In terms of the sequence,this Chinese HCV isolate should belong to HCV group Ⅱ.
