摘要
Anti-hepatoma monoclonal antibodies HAb8,HAb18 IgG and HAb18 F（ab’）<sub>2</sub>were labelled with[<sup>131</sup>I]according to chloramine T method.The labelling rate andradioactivity were 51% and 9.58×10<sup>4</sup>Bq/mg,respectively.After administration of the[<sup>131</sup>I]-labelled conjugates through mouse tail vein,hepatoma xenografts were clearly im-aged,Localization index was 2.15,3.09,and 6.99 for HAb8,HAb18 IgG and HAb18F（ab’）<sub>2</sub>,respectively.A total of 16 hepatoma-bearing nude mice（SMMU-LTNM）were di-vided into 4 groups.When xenografts reached 0.3cm in diameter,the treatment was be-gun by injection of the[<sup>131</sup>I]-conjugates through mouse tail vein.After 2 weeks of treat-ment,obvious inhibitory or killing effects on the xenografts were found.The tu-mor-bearing mice were killed during the period from 14 to 21d after treatment.Xenografts as well as other organs were sampled and viewed under light microscope ac-cording to routine procedure.The vital organs,such as heart,liver,spleen,lung,kidneyand brain showed no pathological lesions.In hepatoma xenografts,some tumor cellmembranes were found,with obscure cytoplasmic structure and increased eosinophilia,representing necrotic alterations.
Anti-hepatoma monoclonal antibodies HAb8,HAb18 IgG and HAb18 F(ab')_2 were labelled with[^(131)I]according to chloramine T method.The labelling rate and radioactivity were 51% and 9.58×10~4Bq/mg,respectively.After administration of the [^(131)I]-labelled conjugates through mouse tail vein,hepatoma xenografts were clearly im- aged,Localization index was 2.15,3.09,and 6.99 for HAb8,HAb18 IgG and HAb18F (ab')_2,respectively.A total of 16 hepatoma-bearing nude mice(SMMU-LTNM)were di- vided into 4 groups.When xenografts reached 0.3cm in diameter,the treatment was be- gun by injection of the[^(131)I]-conjugates through mouse tail vein.After 2 weeks of treat- ment,obvious inhibitory or killing effects on the xenografts were found.The tu- mor-bearing mice were killed during the period from 14 to 21d after treatment. Xenografts as well as other organs were sampled and viewed under light microscope ac- cording to routine procedure.The vital organs,such as heart,liver,spleen,lung,kidney and brain showed no pathological lesions.In hepatoma xenografts,some tumor cell membranes were found,with obscure cytoplasmic structure and increased eosinophilia, representing necrotic alterations.
