摘要
Peripheral blood mononuclear cells（PBMNC）isolated from patients with acuteleukemia（AL）and from normal controls were cultured in a medium containing1000units/ml of recombinant interleukin-2（IL-2）.Marked LAK activity was induced onthe third culture day in the normal controls,with the highest cytotoxicity appearing be-tween day 3 and 5,whereas induction of LAK activity in the AL patients began on the5th day of culture,with a peak level appearing at day 15,showing that the peak ofLAK activity was significantly delayed in AL.LAK cells surface phenotyping tests showedthat CD<sub>8</sub> and CD<sub>16</sub> positive cells began to increase significantly from day 5 and reachedthe highest level at week 3,whereas CD<sub>4</sub><sup>-</sup> subclass began to decrease at day 5 anddropped to the nadir at week 3,indicating that LAK activity was positively correlatedwith the proportion of CD<sub>8</sub><sup>+</sup> and CD<sub>16</sub><sup>+</sup> cells,but negatively with that of CD<sub>4</sub><sup>+</sup> cells.
Peripheral blood mononuclear cells(PBMNC)isolated from patients with acute leukemia(AL)and from normal controls were cultured in a medium containing 1000units/ml of recombinant interleukin-2(IL-2).Marked LAK activity was induced on the third culture day in the normal controls,with the highest cytotoxicity appearing be- tween day 3 and 5,whereas induction of LAK activity in the AL patients began on the 5th day of culture,with a peak level appearing at day 15,showing that the peak of LAK activity was significantly delayed in AL.LAK cells surface phenotyping tests showed that CD_8 and CD_(16) positive cells began to increase significantly from day 5 and reached the highest level at week 3,whereas CD_4^- subclass began to decrease at day 5 and dropped to the nadir at week 3,indicating that LAK activity was positively correlated with the proportion of CD_8^+ and CD_(16)^+ cells,but negatively with that of CD_4^+ cells.
