摘要
A fragment of hepatitis delta antigen(HDAg),a 27-peptide,was synthesized andused to develop an ELISA for the diagnosis of hepatitis delta virus(HDV)infection.It was foundthat positive anti-HD reaction occurred in the 27-peptide coated microtiter well after a sample ofstored serum known to be anti-HD positive was added. Neutralization test revealed that the pep-tide competed with natural HDAg for anti-HD, indicating the similarity of the antigenicity be-tween the peptide and natural HDAg.The peptide was quite specific in its antigenicity withoutcross reaction with normal human or mouse serum or with human anti-HA,anti-HB or anti-HCpositive serum.When the peptide was used clinically,1 anti-HD positive case among 300 blooddonors and 21 anti-HD positive cases among 211 cases of various forms of hepatitis B were detect-ed with the 27-peptide.
A fragment of hepatitis delta antigen(HDAg),a 27-peptide,was synthesized and used to develop an ELISA for the diagnosis of hepatitis delta virus(HDV)infection.It was found that positive anti-HD reaction occurred in the 27-peptide coated microtiter well after a sample of stored serum known to be anti-HD positive was added. Neutralization test revealed that the pep- tide competed with natural HDAg for anti-HD, indicating the similarity of the antigenicity be- tween the peptide and natural HDAg.The peptide was quite specific in its antigenicity without cross reaction with normal human or mouse serum or with human anti-HA,anti-HB or anti-HC positive serum.When the peptide was used clinically,1 anti-HD positive case among 300 blood donors and 21 anti-HD positive cases among 211 cases of various forms of hepatitis B were detect- ed with the 27-peptide.
